Publisher review:PerlPrimer is a free, open-source GUI application written in Perl that designs primers for standard PCR. PerlPrimer is a free, open-source GUI application written in Perl that designs primers for standard PCR, bisulphite PCR, real-time PCR (QPCR) and sequencing. It aims to automate and simplify the process of primer design.PerlPrimer's current features include the following:
- Calculation of possible primer-dimers
- Retrieval of genomic or cdna sequences from Ensembl(including both sequences automatically for QPCR)
- Ability to BLAST search primers using the NCBI server or a local server
- Results can be saved or optionally exported in a tab-delimited format that is compatible with most spreadsheet applications.
- ORF and CpG island detection algorithms
- Ability to add cloning sequences to primers, automatically adjusted to be in-frame
- QPCR primer design without manual intron-exon boundary entry
PerlPrimer calculates primer melting temperature using J. SantaLucia's extensive nearest-neighbour thermodynamic parameters. It allows the user to specify the concentration of Mg2 , dNTPs and primers, or use standard PCR conditions. The result is a highly accurate prediction of primer melting temperature, giving rise to a maximum yeild of product when amplified.
PerlPrimer 1.1.14 is a Perl script for Other scripts design by Owen Marshall.
It runs on following operating system: Linux / BSD.
PerlPrimer is a free, open-source GUI application written in Perl that designs primers for standard PCR.
Operating system:Linux / BSD
PerlPrimer 1.1.14 script details 
