PerlPrimer 1.1.14 Perl script

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    Specification

  • Version: 1.1.14
  • File size: 0 KB
  • File name: perlprimer-1.1.14.zip?download
  • Last update:
  • Platform: Linux / BSD
  • Language: Perl
  • Price:GPL
  • Company: Owen Marshall (View more)

PerlPrimer 1.1.14 script description:



PerlPrimer 1.1.14 is a Perl script for Other scripts design by Owen Marshall. It runs on following operating system: Linux / BSD.
PerlPrimer is a free, open-source GUI application written in Perl that designs primers for standard PCR.

Publisher review:
PerlPrimer is a free, open-source GUI application written in Perl that designs primers for standard PCR. PerlPrimer is a free, open-source GUI application written in Perl that designs primers for standard PCR, bisulphite PCR, real-time PCR (QPCR) and sequencing. It aims to automate and simplify the process of primer design.PerlPrimer's current features include the following: - Calculation of possible primer-dimers - Retrieval of genomic or cdna sequences from Ensembl(including both sequences automatically for QPCR) - Ability to BLAST search primers using the NCBI server or a local server - Results can be saved or optionally exported in a tab-delimited format that is compatible with most spreadsheet applications. - ORF and CpG island detection algorithms - Ability to add cloning sequences to primers, automatically adjusted to be in-frame - QPCR primer design without manual intron-exon boundary entry PerlPrimer calculates primer melting temperature using J. SantaLucia's extensive nearest-neighbour thermodynamic parameters. It allows the user to specify the concentration of Mg2 , dNTPs and primers, or use standard PCR conditions. The result is a highly accurate prediction of primer melting temperature, giving rise to a maximum yeild of product when amplified.
Operating system:
Linux / BSD

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PerlPrimer
Rating: 5.0 out of 5
Based on 1 ratings. 1 user reviews.

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